Isolation and Partial Characterization of Temperature-Sensitive Mutants in Ten Loci That Affect Cell Membrane Synthesis in Escherichia coli: Isolation and Genetic Sorting
AUTOR(ES)
Godson, G. Nigel
RESUMO
Four hundred temperature-sensitive (ts) mutants were isolated by the 3H-glycerol-3-phosphate membrane suicide procedure of Cronan, Ray, and Vagelos and were sorted into 13 groups by the rapid mapping procedures of Low. Recombination and complementation studies on representative members of each group suggested that the ts mutations of all 13 groups are present in genetically different complementation groups. Biochemical studies suggested that 10 of these ts mutations affected cell membrane synthesis. In this paper, the genetic data are presented in detail so that the limits of the Low rapid mapping procedures can be assessed, and in an accompanying paper the partial biochemical characterization of the ts mutations is described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=285296Documentos Relacionados
- Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants
- Isolation of Temperature-Sensitive Membrane Mutants of Escherichia coli K-12
- Temperature-Sensitive Cell-Lethal Mutants of Drosophila: Isolation and Characterization
- Isolation and characterization of temperature-sensitive pantothenate kinase (coaA) mutants of Escherichia coli.
- Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mutants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases