Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.
AUTOR(ES)
Kontis, K J
RESUMO
A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362973Documentos Relacionados
- Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line.
- Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase.
- Escherichia coli Mutants Overproducing Phenylalanyl- and Threonyl-tRNA Synthetase
- Identity elements of tRNA(Thr) towards Saccharomyces cerevisiae threonyl-tRNA synthetase.
- Expression of both Bacillus subtilis threonyl-tRNA synthetase genes is autogenously regulated.