ISOLATION OF ADENYL CYCLASE FROM Escherichia coli*
AUTOR(ES)
Tao, Mariano
RESUMO
We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg++ requirement and is inhibited by fluoride and inorganic pyrophosphate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=534038Documentos Relacionados
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