Isolation of Azospirillum lipoferum 4T Tn5 Mutants Affected in Melanization and Laccase Activity
AUTOR(ES)
Faure, D.
RESUMO
Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed some coloration; the class 2 mutants, which were completely negative for laccase activity, were also colorless. The Tn5 localization on the chromosome or on the cryptic 300-MDa plasmid of A. lipoferum 4T was proven by hybridization for all class 1 mutants or for most class 2 mutants, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201819Documentos Relacionados
- Transposon Mutagenesis of Azospirillum brasilense and Azospirillum lipoferum: Physical Analysis of Tn5 and Tn5-Mob Insertion Mutants
- Isolation of behavioral mutants of Azospirillum brasilense by using Tn5 lacZ.
- Characterization of two hypertransposing Tn5 mutants.
- Randomly induced Escherichia coli K-12 Tn5 insertion mutants defective in hydrogenase activity.
- Regulation of nitrogenase activity by oxygen in Azospirillum brasilense and Azospirillum lipoferum.