Isolation of DNA Strand-Specific Early Messenger RNA Species in Cells Infected by Human Adenovirus 2

AUTOR(ES)

Büttner, Werner

RESUMO

Hybridization to the separated light (L) and heavy (H) strands of adenovirus 2 DNA in 50% formamide at 37° was used to isolate undegraded virus-specific RNA molecules from the polyribosomes of cycloheximide-treated human KB cells early after infection with adenovirus 2. About 20% of polyribosomal RNA labeled with [3H]uridine from 4 to 7 hr after infection was virus-specific. Twice as much labeled RNA was homologous to the L strand as to the H strand. Polyacrylamide gel electrophoresis of RNA selected with unfractionated adenovirus DNA resolved a major component of virus-specific RNA in the 19-20 S region of the gel and smaller amounts of viral RNA in two heterogeneous fractions migrating at 15-18 S and 21-26 S. Selection with individual DNA strands showed that the 19-20 S main size class of early mRNA consists of two homogeneous RNA species with slightly different mobilities, the transcripts from the L and H strand having molecular weights of 7.4 × 105 and 7.7 × 105, respectively. The 15-18 S RNA hybridized with the L strand and the 21-26 S RNA with the H strand.

Documentos Relacionados

• Reassociation of complementary strand-specific adenovirus type 2 DNA with viral DNA sequences of transformed cells.
• Strand-Specific Transcription of Polyoma Virus DNA Early in Productive Infection and in Transformed Cells
• Lack of gene- and strand-specific DNA repair in RNA polymerase III-transcribed human tRNA genes.
• Adenovirus-Associated Virus Multiplication: VI. Base Composition of the Deoxyribonucleic Acid Strand Species and Strand-Specific In Vivo Transcription
• Proteome composition and codon usage in spirochaetes: species-specific and DNA strand-specific mutational biases.