Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
AUTOR(ES)
Morgan, Kelly
FONTE
MyJove Corporation
RESUMO
In the mouse embryo, early hematopoiesis occurs simultaneously in multiple organs, which includes the yolk sac and aorta-gonad-mesonephros region. These regions are crucial in establishing the blood system in the embryos and leads to the eventual movement of stem cells into the fetal liver and then development of adult stem cells in the bonemarrow. Early hematopoietic stem cells can be isolated from these organs through microdissection of the embryo followed by flow cytometric sorting to obtain a more pure population. It remains unclear how these stem cell populations contribute to the fetal and adult stem cell pool. Also, our lab investigates how early stem cells functionally differ from fetal and adult hematopoietic stem cells. Furthermore, our lab sorts different populations of hematopoietic stem cells and test their functional role in the context of a variety of genetic models. In this video, we demonstrate the micro-dissection procedure we commonly use and also show the results of a typical FACS plotfter isolating these rare populations, it is possible to perform a variety of functional assays including: colony assays and bone marrow transplants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2583036Documentos Relacionados
- Identification and characterization of hematopoietic stem cells from the yolk sac of the early mouse embryo.
- Hemopoietic stem cells in murine embryonic yolk sac and peripheral blood.
- In vivo repopulating hematopoietic stem cells are present in the murine yolk sac at day 9.0 postcoitus
- Ventral embryonic tissues and Hedgehog proteins induce early AGM hematopoietic stem cell development
- Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells