Isolation of mutant Saccharomyces cerevisiae strains that survive without sphingolipids.
AUTOR(ES)
Dickson, R C
RESUMO
Sphingolipids comprise a large, widespread family of complex eucaryotic-membrane constituents of poorly defined function. The yeast Saccharomyces cerevisiae is particularly suited for studies of sphingolipid function because it contains a small number of sphingolipids and is amenable to molecular genetic analysis. Moreover, it is the only eucaryote in which mutants blocked in sphingolipid biosynthesis have been isolated. Beginning with a nonreverting sphingolipid-defective strain that requires the addition of the long-chain-base component of sphingolipids to the culture medium for growth, we isolated two strains carrying secondary, suppressor mutations that permit survival in the absence of exogenous long-chain base. Remarkably, the suppressor strains made little if any sphingolipid. A study of how the suppressor gene products compensate for the lack of sphingolipids may reveal the function(s) of these membrane lipids in yeast cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360565Documentos Relacionados
- Cloning and characterization of LCB1, a Saccharomyces gene required for biosynthesis of the long-chain base component of sphingolipids.
- Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains
- Construction of strains of Saccharomyces cerevisiae that grow on lactose.
- Aerobic isolation of an ERG24 null mutant of Saccharomyces cerevisiae.
- Role of inositol-containing sphingolipids in Saccharomyces cerevisiae during inositol starvation.