Isolation of Mycoplasma Membranes by Digitonin
AUTOR(ES)
Rottem, Shlomo
RESUMO
The cell membrane of Mycoplasma hominis was isolated by lysing the cells with digitonin. Electron microscopy and chemical, density gradient, and electrophoretic analyses of the membrane proteins showed the membranes so obtained, like those isolated by osmotic lysis, to be relatively free of cytoplasmic contaminants. Sensitivity to digitonin lysis depended on temperature but was not affected by Mg2+ ions and was only slightly affected by the age of the culture. Accordingly, it seems that digitonin may be used for the isolation of cell membranes from sterol-requiring mycoplasmas that tend to be fairly resistant to osmotic lysis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=247467Documentos Relacionados
- Isolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis.
- Isolation, Characterization, and Immunogenicity of Mycoplasma pneumoniae Membranes
- Isolation of a glycoprotein from Mycoplasma pneumoniae membranes.
- Isolation of Mycoplasma pulmonis membranes and identification of surface antigens.
- New Method for the Isolation of Membranes from Mycoplasma gallisepticum