Isolation of the nitrogen assimilation regulator NRI, the product of the glnG gene of Escherichia coli
AUTOR(ES)
Reitzer, Lawrence J.
RESUMO
The product of the glnG gene, a member of the complex glnALG operon, is an essential component in the response of Escherichia coli K-12 and other enteric bacteria to nitrogen-limited growth. We have purified this protein which we propose to call “NRI,” for nitrogen regulator I, to about 95% purity from an overproducing strain. Purified NRI was identified as a dimer by gel filtration. NRI specifically inhibited initiation of transcription from a DNA fragment containing the glnL promoter but was without effect on lacZ transcription. We determined the intracellular concentration of NRI under different growth conditions by using immunological techniques. The ratio of glutamine synthetase polypeptides, the product of the glnA gene, to NRI polypeptides was about 80:1. NRI was not rapidly degraded after ammonia shock, even though the ability to activate nitrogen-controlled systems was lost.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=384296Documentos Relacionados
- Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.
- Mutations in the glnG gene of Escherichia coli that result in increased activity of nitrogen regulator I.
- Effects of insertions and deletions in glnG (ntrC) of Escherichia coli on nitrogen regulator I-dependent DNA binding and transcriptional activation.
- Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli.
- Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli.