Isolation of vesicular stomatitis virus defective interfering genomes with different amounts of 5'-terminal complementarity.

AUTOR(ES)

Kolakofsky, D

RESUMO

I isolated at least 30 different vesicular stomatitis virus defective interfering (DI) genomes, distinguished by chain length, by five independent undiluted passages of a repeatedly cloned virus plaque. Labeling of the 3' hydroxyl ends of these DI genomes and RNase digestion studies demonstrated that the ends of these DI genomes were terminally complementary to different extents (approximately 46 to 200 nucleotides). Mapping studies showed that the complementary ends of all of the DI genomes were derived from the 5' ends of the nondefective minus-strand genome. Regardless of the extent of terminal complementarity, all of the DI genomes synthesized the same 46-nucleotide minus-strand leader RNA.

Documentos Relacionados

• Vesicular stomatitis virus mutants resistant to defective-interfering particles accumulate stable 5'-terminal and fewer 3'-terminal mutations in a stepwise manner.
• 5′-Terminal Sequence of Vesicular Stomatitis Virus mRNA's Synthesized In Vitro
• In vivo transcription of the 5'-terminal extracistronic region of vesicular stomatitis virus RNA.
• Interference among defective interfering particles of vesicular stomatitis virus.
• Effects of 5'-terminal modifications on the biological activity of defective interfering RNAs of Sindbis virus.