Isotype switching by a microinjected mu immunoglobulin heavy chain gene in transgenic mice.
AUTOR(ES)
Durdik, J
RESUMO
Immunization of transgenic mice carrying an immunoglobulin mu heavy chain resulted in a response dominated by expression of the transgene variable region. Unexpectedly, in a large proportion of the antibody produced by immunized mice, the transgene variable region was associated with IgG rather than IgM. This demonstrates that the transgene can undergo an isotype switch. Four transgenic founder lines all exhibited transgene isotype switching despite the likelihood of random chromosomal integration of the transgene. In addition one of the lines was analyzed by breeding studies and the transgene was found to be genetically unlinked to the immunoglobulin heavy chain (Igh) locus. These results indicate that a precise chromosomal location is not required for isotype switching and suggest the possibility that the isotype switching process can occur interchromosomally.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=286909Documentos Relacionados
- Isotype switching by a microinjected μ immunoglobulin heavy chain gene in transgenic mice
- Human immunoglobulin heavy-chain minilocus recombination in transgenic mice: gene-segment use in mu and gamma transcripts.
- Cell-type-specific and regulated expression of a human gamma 1 heavy-chain immunoglobulin gene in transgenic mice.
- Functional analysis of defined mutations in the immunoglobulin heavy-chain enhancer in transgenic mice.
- A second Xenopus immunoglobulin heavy chain constant region isotype gene