Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis
AUTOR(ES)
Tan, Man-Wah
FONTE
The National Academy of Sciences
RESUMO
We show that a single clinical isolate of the human opportunistic pathogen Pseudomonas aeruginosa (strain PA14), which previously was shown to be pathogenic in mice and plants, also kills Caenorhabditis elegans. The rate of PA14-mediated killing of C. elegans depends on the composition of the agar medium on which PA14 is grown. When PA14 is grown on minimal medium, killing occurs over the course of several days and is referred to as “slow” killing. When PA14 is grown on high-osmolarity medium, killing occurs over the course of several hours and is referred to as “fast” killing. Several lines of evidence, including the fact that heat-killed bacteria are still capable of fast but not slow killing of C. elegans, indicate that fast and slow killing occur by distinct mechanisms. Slow killing involves an infection-like process and correlates with the accumulation of PA14 within worm intestines. Among 10 PA14 virulence-related mutants that had been shown previously to affect pathogenicity in plants and mice, 6 were less effective in killing C. elegans under both fast- and slow-killing conditions, indicating a high degree of commonalty among the P. aeruginosa factors required for pathogenicity in disparate eukaryotic hosts. Thus, we show that a C. elegans pathogenicity model that is genetically tractable from the perspectives of both host and pathogen can be used to model mammalian bacterial pathogenesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=15202Documentos Relacionados
- Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors
- Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis
- Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa
- Pseudomonas aeruginosa PAO1 Kills Caenorhabditis elegans by Cyanide Poisoning
- Quorum-Quenching Acylase Reduces the Virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans Infection Model▿