Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts

AUTOR(ES)

Kullmann , Frank

FONTE

BioMed Central

RESUMO

Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)-polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse transcription PCR, immunohistochemistry and in situ hybridization. CENP-E expression was independent from prednisolone and could not be completely inhibited by serum starvation. RAP-PCR is a suitable method to identify differentially expressed genes in rheumatoid synovial fibroblasts. Also, because motifs of CENP-E show homologies to jun and fos oncogene products and are involved in virus assembly, CENP-E may be involved in the pathophysiology of RA.

Documentos Relacionados

• The Kinesin-like Calmodulin Binding Protein Is Differentially Involved in Cell Division
• Essential role of a kinesin-like protein in Arabidopsis trichome morphogenesis
• Identification of a kinesin-like microtubule-based motor protein in Dictyostelium discoideum.
• Identification of a kinesin-like microtubule-based motor protein in Dictyostelium discoideum
• Origin and Evolution of Kinesin-Like Calmodulin-Binding Protein1[w]