Kinetics of 14C-Photosynthate Uptake by Developing Soybean Fruit

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RESUMO

By pulse-labeling field-grown soybean leaves for 60 seconds at midday with 14CO2 and then sequentially harvesting, dissecting, and extracting the radioactive fruit tissues (of pod and seeds), the route, uptake kinetics, and metabolic fate of 14C-photosynthate as it was imported by 35- to 40-day-old pods were determined. As the [14C]sucrose pulse entered the pods, the seeds became radioactive immediately but a lag of nearly 30 minutes occurred before label could be detected in the pod wall pericarp.

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