Kinetics of the Mating-Specific Aggregation in Saccharomyces cerevisiae

AUTOR(ES)

Campbell, D. A.

RESUMO

Procedures are described for obtaining efficient mating (zygote formation) in the heterothallic yeast Saccharomyces cerevisiae. The methods separate operationally the initial, mating-specific aggregation from subsequent steps in conjugation. This cell-cell interaction has been characterized. The data support the conclusions that (i) aggregation in liquid suspension is a random collision process, and (ii) only non-budded cells participate in aggregation. A mathematical model for the kinetics of aggregation in liquid suspension has been developed which is in good agreement with the experimental data.

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