Kinetics of thymidine incorporation into detergent-soluble DNA of mouse lymphocytes.

AUTOR(ES)

Strauss, P R

RESUMO

We reported recently that splenocytes from concanavalin A-stimulated mice rapidly incorporated [3H]thymidine into non-mitochondrial DNA that was detergent soluble and distributed in size classes between 200 and 5000 base pairs. In this report we show that small [3H]thymidine-labeled oligonucleotides (less than 100 base pairs long) appear by 15 min. Subsequently, [3H]thymidine in small oligonucleotides diminishes as incorporation into larger size classes of detergent-soluble DNA occurs in a pattern that is stable for at least 3 hr. Although incorporation of [3H]thymidine into the oligonucleotides is not sensitive to aphidicolin or hydroxyurea, the appearance of [3H]thymidine in larger species is blocked by both drugs. These results indicate that the enzymatic process involved in synthesis of oligonucleotides is somehow distinct from the process involved in synthesis of larger detergent-soluble size classes. Synthesis of the latter may be replication related.

Documentos Relacionados

• Role for topoisomerases in the release of DNA into the detergent-soluble fraction of eukaryotic cells.
• Reconstitution of purified detergent-soluble HLA-A and HLA-B antigens into phospholipid vesicles.
• Quinolones affect thymidine incorporation into the DNA of human lymphocytes.
• Partial Purification of Detergent-Soluble HL-A Antigen and Its Cleavage by Papain
• The Detergent-Soluble Maltose Transporter Is Activated by Maltose Binding Protein and Verapamil