Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells
AUTOR(ES)
Pierce, Andrew J.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Chromosomal double-strand breaks (DSBs) in mammalian cells are repaired by either homology-directed repair (HDR), using a homologous sequence as a repair template, or nonhomologous end-joining (NHEJ), which often involves sequence alterations at the DSB site. To characterize the interrelationship of these two pathways, we analyzed HDR of a DSB in cells deficient for NHEJ components. We find that the HDR frequency is enhanced in Ku70−/−, XRCC4−/−, and DNA-PKcs−/− cells, with the increase being particularly striking in Ku70−/− cells. Neither sister-chromatid exchange nor gene-targeting frequencies show a dependence on these NHEJ proteins. A Ku-modulated two-ended versus one-ended chromosome break model is presented to explain these results.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=312854Documentos Relacionados
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