Lability of human creatine kinase isoenzymes at 37 degrees C: a complication of electrophoretic separation.
AUTOR(ES)
Nealon, D A
RESUMO
The activity of the brain specific isoenzyme of creatine kinase is shown to fall off rapidly at 37 degrees C, particularly in the presence of albumin. Dithiothreitol cannot reverse this lability. The implications of this finding suggest that electrophoretic techniques which use incubation methods to detect the brain specific isoenzyme of creatine kinase may underestimate the true activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=475871Documentos Relacionados
- Protein kinase C: a family of isoenzymes with distinct roles in pathogenesis
- Isoenzymes of protein kinase C: differential involvement in apoptosis and pathogenesis.
- Spoilage of chicken skin at 2 degrees C: electron microscopic study.
- Conversion of Blastomyces dermatitidis to the yeast form at 37 degrees C and 26 degrees C.
- A morphometric study of endothelial cells of human corneas stored in MK media and warmed at 37 degrees C.