Lack of Regulation of the Modification-Dependent Restriction Enzyme McrBC in Escherichia coli

AUTOR(ES)

Murphy, Mark

FONTE

American Society for Microbiology

RESUMO

Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.

Documentos Relacionados

• Evidence of participation of McrB(S) in McrBC restriction in Escherichia coli K-12.
• Valyl-tRNA synthetase modification-dependent restriction of bacteriophage T4.
• Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12.
• Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.
• The McrBC restriction endonuclease assembles into a ring structure in the presence of G nucleotides