Lesão por esmagamento do nervo isquiático de ratos: estudo da vascularização / Crush injury of the rat sciatic nerve: vascularization study.

AUTOR(ES)

Celia Aparecida Stellutti Pachioni

DATA DE PUBLICAÇÃO

2006

RESUMO

The objective of this work was to study the acute intraneurial microvascular changes in the rat sciatic nerve submitted to a crush injury by different loads. Sixty Wistar male rats were used and distributed into two experimental groups according to vessel injection protocol and subdivided according to the crush load. The right sciatic nerves of each experimental group were isolated and submitted to crush by different loads (0,5 Kg, 1 Kg, 5 Kg, 10 kg and 15 kg) for ten minutes. The left sciatic nerves were used as controls. After the crush, thirty animals were submitted to Protocol I, which consisted of: abdominal aorta catheterization, manual perfusion withf a solution composed of China ink and gelatin 5% in formaldehyde 10%. After that the right and left nerves were collected, fixed in formaldehyde10%, dehydrated and diaphanized for longitudinal analysis of the intraneurial vessels. The other thirty animals were submitted to Protocol II, which consisted of: abdominal aorta catheterization, as described above and perfused with a solution of China ink and gelatin 5% in physiologic saline and then placed in a freezer at -20°C for one hour. After that the nerves were dissected and removed in their entire length, cut into three fragments, frozen in isopentane and dry ice and placed in a freezer at -70°C, cut in semi-serial histological transverse sections for analysis and intraneurial vessel quantification.The results showed endoneurial and epineurial haematoma areas in the different groups, indicating that the crush forces were enough to damage the intraneurial vessels, specially with high loads. The morphometrical analysis showed a different profile in the three fragments, with smaller number of vessels in the crush region then above and below, suggesting that the damage to intraneurial vessels was proportional to the crush load, causing endoneurial and epineurial haematoma, which creates an unfavorable microenvironment for the regeneration of the nerve fibers that were also damaged in that model.

ASSUNTO(S)

sciatic nerve vascularização esmagamento rat crush injury vascularization nervo isquiático rato

Documentos Relacionados

• Lesão por esmagamento do nervo isquiático de ratos: estudo da vascularização
• Correlação entre diferentes métodos de avaliação funcional da marcha de ratos com lesão por esmagamento do nervo isquiático
• Índice funcional do ciático nas lesões por esmagamento do nervo ciático de ratos. Avaliação da reprodutibilidade do método entre examinadores
• MORFOLOGIA DO NERVO ISQUIÁTICO DE RATOS SUBMETIDOS À IMOBILIZAÇÃO DE PATA
• Os efeitos do ultra-som terapêutico nas lesões por esmagamento do nervo ciático de ratos: análise funcional da marcha