Leukotriene C promotes prostacyclin synthesis by human endothelial cells.
AUTOR(ES)
Cramer, E B
RESUMO
Cultured endothelial cells from human umbilical vein were labelled with [3H]arachidonic acid for 16 hr. The radiolabel was localized primarily in phospholipids (93%) and 73% was distributed equally between phosphatidylcholine and phosphatidylethanolamine. Leukotriene C (10-1,000 nM) promoted a dose-dependent release of radiolabel into the culture medium. This response was 3.3 times control values at 100 nM. The major arachidonic acid metabolite synthesized was prostacyclin, which was 33% of the total released radiolabel. Endothelial cells also released small amounts of prostaglandin F2 alpha (6.1%), unidentified lipoxygenase products (14.8%), and unreacted arachidonic acid (33%). The 30-min time course of release was independent of the leukotriene C concentration used. Leukotriene D at similar concentrations also promoted endothelial cells to release primarily prostacyclin and unreacted arachidonic acid. The release of prostacyclin, a potent vasodilator agent, may be an important mediator in slow reacting substance effects on the vasculature.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=394210Documentos Relacionados
- Islet-activating protein inhibits leukotriene D4- and leukotriene C4- but not bradykinin- or calcium ionophore-induced prostacyclin synthesis in bovine endothelial cells.
- Synthesis of prostaglandin I2 (prostacyclin) by cultured human and bovine endothelial cells.
- Nitroglycerin Stimulates Synthesis of Prostacyclin by Cultured Human Endothelial Cells
- Synthesis of Prostacyclin from Platelet-derived Endoperoxides by Cultured Human Endothelial Cells
- Effect of fatty acid modification on prostacyclin production by cultured human endothelial cells.
