Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

AUTOR(ES)

Merjan, A.J., Kanashiro, C.A., Krieger, J.E., Han, S.W., Paiva, A.C.M.

FONTE

Brazilian Journal of Medical and Biological Research

DATA DE PUBLICAÇÃO

2001-09

RESUMO

A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.

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