Linkage Analysis in Dictyostelium discoideum Using Temperature-Sensitive Growth Mutants Selected with Bromodeoxyuridine
AUTOR(ES)
Kessin, Richard H.
RESUMO
Amoebae of the cellular slime mold Dictyostelium discoideum grown in the presence of bromodeoxyuridine are killed on exposure to near-ultraviolet light. By using this phenomenon, a method was devised by which mutants of D. discoideum that are temperature-sensitive for growth can be readily obtained. Three such mutants have been characterized genetically and each was found to be associated with a different linkage group. Two of these linkage groups have not previously been described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=245681Documentos Relacionados
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