Lipid peroxidation and nitric oxide inactivation in postmenopausal women

AUTOR(ES)

Pereira, Isabela Rosier Olimpio, Bertolami, Marcelo Chiara, Faludi, André Arpad, Campos, Maria Fernanda, Ferderbar, Simone, Lima, Emersom Silva, Aldrighi, José Mendes, Abdalla, Dulcineia Saes Parra

FONTE

Arquivos Brasileiros de Cardiologia

DATA DE PUBLICAÇÃO

2003-04

RESUMO

OBJECTIVE: To assess the effect of endogenous estrogens on the bioavailability of nitric oxide (·NO) and in the formation of lipid peroxidation products in pre- and postmenopausal women. METHODS: NOx and S-nitrosothiols were determined by gaseous phase chemiluminescence, nitrotyrosine was determined by ELISA, COx (cholesterol oxides) by gas chromatography, and cholesteryl linoleate hydroperoxides (CE18:2-OOH), trilinolein (TG18:2-OOH), and phospholipids (PC-OOH) by HPLC in samples of plasma. RESULTS: The concentrations of NOx, nitrotyrosine, COx, CE18:2-OOH, and PC-OOH were higher in the postmenopausal period (33.8±22.3 mM; 230±130 nM; 55±19 ng/mL; 17±8.7 nM; 2775±460 nM, respectively) as compared with those in the premenopausal period (21.1±7.3 mM; 114±41 nM; 31±13 ng/mL; 6±1.4 nM; 1635±373 nM). In contrast, the concentration of S-nitrosothiols was lower in the postmenopausal period (91±55 nM) as compared with that in the premenopausal p in the premenopausal period (237±197 nM). CONCLUSION: In the postmenopausal period, an increase in nitrotyrosine and a reduction of S-nitrosothiol formation, as well as an increase of COx, CE18:2-OOH and PC-OOH formation occurs. Therefore, •NO inactivation and the increase in lipid peroxidation may contribute to endothelial dysfunction and to the greater risk for atherosclerosis in postmenopausal women.

Documentos Relacionados

• BN 80933, a dual inhibitor of neuronal nitric oxide synthase and lipid peroxidation: A promising neuroprotective strategy
• CONCURRENT TRAINING AND TAURINE IMPROVE LIPID PROFILE IN POSTMENOPAUSAL WOMEN
• Peak expiratory nitric oxide differences in men and women: relation to the menstrual cycle.
• Free Radicals, Lipid Peroxidation and Cancer
• Nitric oxide production and inducible nitric oxide synthase expression in inflammatory arthritides.