Lithium phthalocyanine: a probe for electron paramagnetic resonance oximetry in viable biological systems.
AUTOR(ES)
Liu, K J
RESUMO
Lithium phthalocyanine (LiPc) is a prototype of another generation of synthetic, metallic-organic, paramagnetic crystallites that appear very useful for in vitro and in vivo electron paramagnetic resonance oximetry. The peak-to-peak line width of the electron paramagnetic resonance spectrum of LiPc is a linear function of the partial pressure of oxygen (pO2); this linear relation is independent of the medium surrounding the LiPc. It has an extremely exchange-narrowed spectrum (peak-to-peak line width = 14 mG in the absence of O2). Physicochemically LiPc is very stable; its response to pO2 does not change with conditions and environments (e.g., pH, temperature, redox conditions) likely to occur in viable biological systems. These characteristics provide the sensitivity, accuracy, and range to measure physiologically and pathologically pertinent O2 tensions (0.1-50 mmHg; 1 mmHg = 133 Pa). The application of LiPc in biological systems is demonstrated in measurements of pO2 in vivo in the heart, brain, and kidney of rats.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=46735Documentos Relacionados
- Soft x-ray magnetic circular dichroism: a probe for studying paramagnetic bioinorganic systems.
- An Electron Paramagnetic Resonance Spin-Probe Study of Membrane-Permeability Changes with Seed Aging.
- Electron paramagnetic resonance of chlorophyll-water aggregates.
- Sticky ions in biological systems.
- Use of Fe3+ ion probe to study intensively weathered soils utilizing electron paramagnetic resonance and optical spectroscopy
