Localization of acyl carrier protein in Escherichia coli.

AUTOR(ES)

Jackowski, S

RESUMO

Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). Moreover, bacterial membranes did not bind a significant amount of acyl carrier protein or its thioesters in vitro. A thioesterase activity specific for long-chain acyl-acyl carrier protein was associated with the inner membrane.

Documentos Relacionados

• Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli.
• Activation of long chain fatty acids with acyl carrier protein: demonstration of a new enzyme, acyl-acyl carrier protein synthetase, in Escherichia coli.
• Mixed disulfides of acyl carrier protein and coenzyme A with specific soluble proteins in Escherichia coli.
• An essential function for acyl carrier protein in the biosynthesis of membrane-derived oligosaccharides of Escherichia coli.
• Inhibition of Escherichia coli Acetyl Coenzyme A Carboxylase by Acyl-Acyl Carrier Protein