Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site
AUTOR(ES)
Park, Hay-Oak
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Yeast cells of different cell type exhibit distinct budding patterns that reflect the organization of the actin cytoskeleton. Bud1p (Rsr1p), a Ras-like GTPase, and Bud2p, a GTPase-activating protein for Bud1p, are essential for proper budding pattern. We show that Bud2p is localized at the presumptive bud site in G1 cells in all cell types and that this localization is independent of Bud1p. Bud2p subsequently localizes to the mother-bud neck after bud emergence; this localization depends on the integrity of the septins. These observations indicate that Bud2p becomes positioned in G1 cells by recognizing cell type-specific landmarks at the presumptive bud site.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316924Documentos Relacionados
- IRA2, an upstream negative regulator of RAS in yeast, is a RAS GTPase-activating protein.
- Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1.
- GTPase-Activating Proteins for Cdc42
- Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe.
- Yeast Rab GTPase-activating Protein Gyp1p Localizes to the Golgi Apparatus and Is a Negative Regulator of Ypt1p