Localization of Escherichia coli rpoC Mutations That Affect RNA Polymerase Assembly and Activity at High Temperature

AUTOR(ES)

Nedea, Eduard C.

FONTE

American Society for Microbiology

RESUMO

We localized five rpoC (β′) mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed β′ residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpb1-1. Thus, distinct, predominantly conserved β′ residues participate in interactions holding RNA polymerase together.

Documentos Relacionados

• Cyanobacterial RNA polymerase genes rpoC1 and rpoC2 correspond to rpoC of Escherichia coli.
• Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2.
• Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC).
• The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.
• A missense mutation in the rpoC gene affects chromosomal replication control in Escherichia coli.