Localization of the estrogen-binding site of alpha-fetoprotein in the chimeric human-rat proteins.
AUTOR(ES)
Nishi, S
RESUMO
Rat alpha-fetoprotein (AFP) has been demonstrated to bind estrogen, whereas human AFP lacks the activity. We constructed four chimeric molecules from cDNAs encoding these AFPs with the use of two restriction sites common to them and expressed them as well as rat and human AFP cDNA in yeast. The recombinant molecules were purified, characterized, and found to have the predicted structures. Analyses of estrogen binding indicated that a rat AFP sequence composed of residues 423-506 that contains 31 rat-specific amino acids is essential for the activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=51393Documentos Relacionados
- A low-affinity estrogen-binding site in pregnant rat uteri: analysis and partial purification.
- Alpha-fetoprotein screening.
- Developmental regulation of albumin and alpha-fetoprotein gene expression in the rat.
- DNaseI sensitivity of the rat albumin and alpha-fetoprotein genes.
- Hormonal modulation of alpha-fetoprotein gene expression in newborn rat livers.