Localization of the Stringent Protein of Escherichia coli on the 50S Ribosomal Subunit
AUTOR(ES)
Ramagopal, S.
RESUMO
The “stringent” protein of the ribosome, required for its synthesis of (p)ppGpp, is readily lost during zonal centrifugation. However, enough can be retained to permit its qualitative localization. It is found in native 50S subunits, runoff 70S ribosomes, and polysomes, but not in native 30S subunits. This protein, therefore, appears to be attached to the 50S moiety of the ribosome, and it may be a constant (though easily removed) component of that structure rather than a factor that joins and leaves during the ribosome cycle.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388106Documentos Relacionados
- Asymmetry in the 50S Ribosomal Subunit of Escherichia coli
- Asymmetry in the 50S ribosomal subunit of Escherichia coli
- Mutation affecting the thermolability of the 50S ribosomal subunit in Escherichia coli.
- Cooperative interactions among protein and RNA components of the 50S ribosomal subunit of Escherichia coli.
- Location of eight ribosomal proteins on the surface of the 50S subunit from Escherichia coli.
