Location of platinum binding sites on bacteriorhodopsin by electron diffraction

AUTOR(ES)

Dumont, Mark E.

RESUMO

A platinum-containing derivative of bacteriorhodopsin has been prepared by treating purple membranes with glycyl-L-methionatoplatinum. Low-dose electron diffraction was used to identify Pt binding sites in the 5.6 Å resolution reconstruction of the bacteriorhodopsin unit cell in projection. This is a necessary first step in the use of the Pt derivative for identifying the parts of the amino acid sequence corresponding to the α helices in the bacteriorhodopsin structure and for obtaining phases for reflections out to 3.5 Å resolution by the method of heavy-atom isomorphous replacement. The largest peak in a Fourier difference map between platinum-labeled and native purple membrane is larger than the spurious features expected to arise from errors in measurements of diffraction intensities.

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