Long-distance movement factor: a transport function of the potyvirus helper component proteinase.
AUTOR(ES)
Cronin, S
RESUMO
Transport of viruses from cell to cell in plants typically involves one or more viral proteins that supply dedicated movement functions. Transport from leaf to leaf through phloem, or long-distance transport, is a poorly understood process with requirements differing from those of cell-to-cell movement. Through genetic analysis of tobacco etch virus (TEV; potyvirus group), a novel long-distance movement factor was identified that facilitates vascular-associated movement in tobacco. A mutation in the central region of the helper component proteinase (HC-Pro), a TEV-encoded protein with previously described activities in aphid-mediated transmission and polyprotein processing, inactivated long-distance movement. This mutant virus exhibited only minor defects in genome amplification and cell-to-cell movement functions. In situ histochemical analysis revealed that the mutant was capable of infecting mesophyll, bundle sheath, and phloem cells within inoculated leaves, suggesting that the long-distance movement block was associated with entry into or exit from sieve elements. The long-distance movement defect was specifically complemented by HC-Pro supplied in trans by a transgenic host. The data indicate that HC-Pro functions in one or more steps unique to long-distance transport.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=160803Documentos Relacionados
- Leader Proteinase of Beet Yellows Virus Functions in Long-Distance Transport
- VPg of tobacco etch potyvirus is a host genotype-specific determinant for long-distance movement.
- Interaction between Long-Distance Transport Factor and Hsp70-Related Movement Protein of Beet Yellows Virus
- Long-Distance Water Transport in Aquatic Plants.
- Destination-Selective Long-Distance Movement of Phloem ProteinsW⃞