Long Fluorescence Lifetime Molecular Probes Based on Near Infrared Pyrrolopyrrole Cyanine Fluorophores for In Vivo Imaging
AUTOR(ES)
Berezin, Mikhail Y.
FONTE
The Biophysical Society
RESUMO
Fluorescence lifetime (FLT) properties of organic molecules provide a new reporting strategy for molecular imaging in the near infrared (NIR) spectral region. Unfortunately, most of the NIR fluorescent dyes have short FLT typically clustered below 1.5 ns. In this study, we demonstrate that a new class of NIR fluorescent dyes, pyrrolopyrrole cyanine dyes, have exceptionally long FLTs ranging from 3 to 4 ns, both in vitro (dimethyl sulfoxide and albumin/water solutions) and in vivo (mice). These results provide a new window for imaging molecular processes, rejecting backscattered light and autofluorescence, and multiplexing imaging information with conventional NIR fluorescent dyes that absorb and emit light at similar wavelengths.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2770609Documentos Relacionados
- Near-infrared-emissive polymersomes: Self-assembled soft matter for in vivo optical imaging
- Video-assisted thoracoscopic thoracic duct ligation with near-infrared fluorescence imaging with indocyanine green
- Noninvasive imaging of the distribution in oxygen in tissue in vivo using near-infrared phosphors.
- Editorial Comment: Best practices in near-infrared fluorescence imaging with indocyanine green (NIRF/ICG)-guided robotic urologic surgery: a systematic review-based expert consensus
- Fluorescence lifetime imaging by asynchronous pump-probe microscopy.
