Loss of R Factors Promoted by Bacteriophage M13 and the M13 Carrier State

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RESUMO

The infection of different Hfr strains of Escherichia coli bearing derepressed R factors of the fi+ or fi− type can result in the loss of the R factor and the conversion of the infected cells to the R− state. This extends earlier observations on the elimination of F′ factors by bacteriophage M13 infection. Variability in the efficiency of this conversion can arise because of genetic factors independent of the R factor being eliminated. A fraction of the infected but unconverted R+ cells were M13 carrier strains. The carrier state had an intracellular basis, and single R+ cells could maintain the carrier state.

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