Lysine-Ketoglutarate Reductase Activity in Developing Maize Endosperm 1
AUTOR(ES)
Arruda, Paulo
RESUMO
Lysine-ketoglutarate reductase activity was detected and characterized in the developing endosperm of maize (Zea mays L.). The enzyme showed specificity for its substrates: lysine, α-ketoglutarate, and NADPH. Formation of the reaction product saccharopine was demonstrated. The pH optimum of the enzyme was close to 7, and the Km for lysine and α-ketoglutarate were 5.2 and 1.8 millimolar, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426341Documentos Relacionados
- Familial hyperlysinemia with lysine-ketoglutarate reductase insufficiency
- Purification and Characterization of Bifunctional Lysine-Ketoglutarate Reductase/Saccharopine Dehydrogenase from Developing Soybean Seeds1
- Purification and Characterization of the Bifunctional Enzyme Lysine-Ketoglutarate Reductase-Saccharopine Dehydrogenase from Maize.
- Regulation of lysine catabolism through lysine-ketoglutarate reductase and saccharopine dehydrogenase in Arabidopsis.
- A T-DNA Insertion Knockout of the Bifunctional Lysine-Ketoglutarate Reductase/Saccharopine Dehydrogenase Gene Elevates Lysine Levels in Arabidopsis Seeds1
