MaGiK Method of T-Cell Receptor Repertoire Analysis
AUTOR(ES)
Killian, M. Scott
FONTE
American Society for Microbiology
RESUMO
T-cell receptor diversity enables the cellular immune response to recognize a broad range of viral and other pathogenic agents. An increasingly common method of characterizing T-cell receptor diversity and usage in response to antigenic challenges involves the identification of clonal expansions by PCR amplification of the CDR3 region of distinct TCRVβ families. Though clonal expansions often appear evident upon visual inspection of the results, a systematic method is needed for the valid enumeration of these expansions. Here, we describe a novel analysis method, termed the MaGiK method, for systematically identifying and enumerating clonal T-cell expansions and for applying the results to investigations of the T-cell receptor repertoire.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=120035Documentos Relacionados
- Limitations in plasticity of the T-cell receptor repertoire.
- Biased T-Cell Antigen Receptor Repertoire in Lyme Arthritis
- Generation of the alloreactive T-cell repertoire: interaction of T-cell genotype and maturation environment.
- Gamma delta T-cell receptor repertoire in acute multiple sclerosis lesions.
- Nondeletional T-Cell Receptor Transgenic Mice: Model for the CD4+ T-Cell Repertoire in Chronic Hepatitis B Virus Infection
