Mapeamento de epÃtopos das proteÃnas estruturais VP1 e VP4 do vÃrus da hepatite A reconhecidos por cÃlulas T

AUTOR(ES)

Karla Patricia de Sousa Barbosa

DATA DE PUBLICAÇÃO

2009

RESUMO

Hepatitis A infection is found all over the world, and high prevalence is associated with poor socioeconomic conditions and diverse epidemiological patterns. The epitope mapping of hepatitis A virus (HAV) can to contribute with the development of a potentially safer vaccine against HAV and the identification of HLA binding molecules is important for both understanding the basing molecular function of the immune system and for vaccine development. The aim of the present investigation was to identify T cell epitopes in the structural proteins VP1 and VP4 in volunteers vaccinated or infected naturally for HAV. For mapping immunidominant epitopes, seventy peptides (each with 15-mers, overlap of 11) were synthesized (Shaffer - USA), covering the 345 amino acids (aa) of the VP1 and 23 aa of VP4 proteins, of the strain HM 175. These peptides were tested by ELISPOT with PBMCs of volunteer groups. The results showed that human PBMC reacted with the eighteen peptides from VP1. However, in vaccinated group, the peptides more immunogenic were distributed at amino acid positions 33-47, 45-59, 49-63, 73-87 and 153-167, whereas in the infected group the peptides more immunogenic were 1-15, 49-63, 69-83 and 149-163. In HLA study, we found the alelos that showed high frequency in studied groups: HLA A2, HLA B40, HLA Cw7, HLA DR11 and HLA DQ6. Our results are similar within LaViTEâs coorte. Thus, our study identified several reactive epitopes on the VP1 and VP4 proteins of HAV. This information may be of significantly relevant for the development of potential epitope-based vaccines that recognized by T cells restricted by human HLA alleles.

ASSUNTO(S)

epÃtopos de linfÃcito t peptides ciencias biologicas hepatitis a t lymphocyte epitopes peptÃdeos hepatite a

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