Mapping and 5' end determination of kinetoplast maxicircle gene transcripts from Leishmania tarentolae.

AUTOR(ES)

Simpson, A M

RESUMO

Transcripts for six Leishmania tarentolae maxicircle structural genes (cytochrome oxidase subunits I, II and III, cytochrome b, human mitochondrial unidentified reading frames 4 and 5) and several unidentified open reading frames were mapped, and the locations of the 5' ends determined by primer runoff analysis. All genes studied here are transcribed from the same strand as the 12S and 9S ribosomal RNAs except for the cytochrome oxidase subunit I gene. In two cases (ORF3 and ORF4, ORF5 and ORF6), a single transcript covers two contiguous overlapping reading frames. The 5' ends of the RNAs are located 20-64 nt from the putative translation initiation codons. Primary transcripts from a mitochondrial RNA preparation were 5' end-labeled with guanylyltransferase and alpha -32P-GTP; the major labeled species comigrated with the 12S and 9S mitochondrial rRNAs, and in addition there were at least four higher molecular weight labeled species.

Documentos Relacionados

• A minimal ribosomal RNA: sequence and secondary structure of the 9S kinetoplast ribosomal RNA from Leishmania tarentolae.
• The divergent region of the Leishmania tarentolae kinetoplast maxicircle DNA contains a diverse set of repetitive sequences.
• Independent 5' and 3'-end determination of multiple dihydrofolate reductase transcripts.
• Comparison of maxicircle DNAs of Leishmania tarentolae and Trypanosoma brucei.
• Role of the locus and of the resistance gene on gene amplification frequency in methotrexate resistant Leishmania tarentolae.