Mapping Chromosomal Genes of SACCHAROMYCES CEREVISIAE Using an Improved Genetic Mapping Method
AUTOR(ES)
Wickner, Reed B.
RESUMO
A triploid (3n) strain of Saccharomyces cerevisiae was constructed carrying a standard marker on each of chromosomes I through XVII in the -/+/+ configuration. This is called a "supertriploid." Meiotic spores from this strain (n + ∼ n/2) were mated with a haploid (n) carrying an unmapped mutation. Meiotic analysis of each zygote clone (2n + ∼ n/2) produced in this way resulted in elimination of an average of 4.2 chromosomes as the possible location of the unmapped marker. The distribution of extra chromosomes in the 2n + ∼ n/2) strains was nearly random. Meiotic segregants of these crosses carrying the unmapped mutation in the -/+ configuration were then crossed with multiply marked haploid strains to further narrow the possible location of the unmapped mutation to a single chromosome. Scoring of markers by complementation tests was simplified by mating spore clones with mixtures of a and α strains, each pair carrying the same set of markers. Using this new, more rapid method ("supertriploid mapping"), eight genes required for the maintenance of the killer plasmid were located on the genetic map of S. cerevisiae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1214038Documentos Relacionados
- A Mapping Method for SACCHAROMYCES CEREVISIAE Using rad52-Induced Chromosome Loss
- Genetic mapping of Ty elements in Saccharomyces cerevisiae.
- An improved method for photofootprinting yeast genes in vivo using Taq polymerase.
- Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae.
- Discovery of estrogen-responsive genes using an improved method which combines subtractive hybridization and PCR.