Mapping of immunoreactive sites of pneumococcal pneumolysin by use of synthetic peptides.

AUTOR(ES)

Salo, P

RESUMO

In the search for better diagnostic tools and improved vaccines against pneumococcal diseases, continuous antigenic sites of the pneumococcal pneumolysin molecule were identified with partially overlapping synthetic peptides. Peptides were derived from the predicted amino acid sequence according to the known DNA sequence of pneumolysin. The immunoreactivities of the peptides were studied by enzyme immunoassay with seven human serum samples and three rabbit hyperimmune serum samples. Two immunoreactive sites in the overlapping peptides at the amino-terminal end of the pneumolysin molecule were identified in this study. Two common immunoreactive sites for both human and rabbit sera were demonstrated.

Documentos Relacionados

• Mapping antigenic sites on the major outer membrane protein of Chlamydia trachomatis with synthetic peptides.
• Mapping of T cell epitopes using recombinant antigens and synthetic peptides.
• Affinity purification of synthetic peptides.
• Lack of tuberculin activity of synthetic peptides.
• Characterization of murine B-cell epitopes on the Mycobacterium leprae proline-rich antigen by use of synthetic peptides.