Mapping of two isoleucine tRNA isoacceptor genes in bacteriophage T5 DNA.
AUTOR(ES)
Hunt, C
RESUMO
T5 bacteriophage codes for the synthesis of more than 14 different tRNA species, which map in four separate clusters in the C segment of the T5 chromosome. In this study, two tRNAile isoacceptor species have been identified by reverse-phase chromatography and shown to be transcribed from two different tRNA loci along the T5 chromosome. The map positions of the tRNA isoacceptors were aided by the use of several T5 deletion mutants in which the position and size of the deleted DNA segments had been previously determined by heteroduplex mapping. Hybridization analysis suggests the presence of some sequence homology between the two tRNAile species.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354966Documentos Relacionados
- Identification and location of nine T5 bacteriophage tRNA genes by DNA sequence analysis.
- Nucleotide sequence of the phage T5 DNA segment containing six tRNA genes.
- Distribution of "minor" nicks in bacteriophage T5 DNA.
- New physical map of bacteriophage T5 DNA.
- Nucleotide sequence of the bacteriophage T5 DNA fragment containing a distal part of tRNA gene region.
