Maternal transmission of P element transposase activity in Drosophila melanogaster depends on the last P intron
AUTOR(ES)
Simmons, Michael J.
FONTE
The National Academy of Sciences
RESUMO
Maternal transmission of RNAs or proteins through the egg cytoplasm plays an important role in eukaryotic development. We show that the transposase activity encoded by the P transposable element of Drosophila melanogaster is transmitted through the oocytes of females heterozygous for this element even when these oocytes do not carry the element itself. However, this maternal transmission is abolished when the last of three introns is removed from the P element. These facts imply that maternal transmission of transposase activity involves the RNA transcribed from the P element rather than the polypeptide it encodes, and that to be transmitted maternally, this RNA must possess the last intron. Examination of the intron's sequence reveals that it contains a motif of nine nucleotides that has been implicated in the maternal transmission of developmentally significant RNAs. This same intron limits expression of the P transposase to the germ line of Drosophila. Thus, the last P intron has two important biological functions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123136Documentos Relacionados
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