MCM1 binds to a transcriptional control element in Ty1.
AUTOR(ES)
Errede, B
RESUMO
Some Ty1 transposable-element insertion mutations of Saccharomyces cerevisiae activate adjacent-gene expression. These Ty1-activated genes are regulated similarly to certain mating genes. This report shows that the MCM1 protein, which binds to several mating genes, also binds to a transcriptional regulatory sequence in Ty1. The binding of MCM1 to Ty1 correlates with the ability of its binding site to function as a component of the Ty1 transcriptional activator. This correlation supports the idea that MCM1 is important for Ty1-activated gene expression. At mating-gene promoters, MCM1 binds with coactivators or repressors such as STE12, alpha 1, or alpha 2. In contrast, MCM1 binds without these associated DNA-binding proteins at its site in Ty1. This finding suggests that its role in Ty1-mediated transcription is different from that at mating genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=358884Documentos Relacionados
- SPT13 (GAL11) of Saccharomyces cerevisiae negatively regulates activity of the MCM1 transcription factor in Ty1 elements.
- Nuclear factor EF-1A binds to the adenovirus E1A core enhancer element and to other transcriptional control regions.
- Plus-strand strong-stop DNA synthesis in retrotransposon Ty1.
- Mcm1 is required to coordinate G2-specific transcription in Saccharomyces cerevisiae.
- Chromosome loss, hyperrecombination, and cell cycle arrest in a yeast mcm1 mutant.