Mechanism of Glycolate Transport in Spinach Leaf Chloroplasts 1
AUTOR(ES)
Takabe, Tetsuko
RESUMO
The incorporation of 14CO2 into glycolate by intact spinach leaf (Spinacia oleracea L. var. Kyoho) chloroplasts exposed to 14CO2 (NaH14CO3, 1 millimolar) in the light was determined as a function of O2 concentrations in the reaction media. A hyperbolic saturation curve was obtained, apparent Km (O2) of 0.28 millimolar, indicating that glycolate is produced predominantly by ribulose-1,5-bisphosphate carboxylase/oxygenase. A concentration gradient of glycolate was invariably observed between chloroplast stroma and the outside media surrounding chloroplasts during photosynthetic 14CO2 fixation under an O2 atmosphere.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426051Documentos Relacionados
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