Mechanism of nuclear DNA replication in radicles of germinating cotton
AUTOR(ES)
Clay, Willard F.
RESUMO
The mechanism of nuclear DNA replication in radicles of germinating cotton (Gossypium barbadense) was investigated. Pulse-labeling with [3H]thymidine indicates that replication intermediates are of small molecular weight (4-10S) and behave as if single-stranded. Prolonged labeling indicates that intermediates are of discrete size, suggesting a mechanism of discontinuous replication. Electron microscopy of nuclear DNA demonstrates a complex architecture which may include “eye forms” and “forks” similar to those reported in prokaryotes and animal systems. The possible universality of DNA replication mechanisms is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=430984Documentos Relacionados
- Size and Specific Radioactivity of the [3H]Thymidine Triphosphate Pool and DNA Synthesis in the Radicles of Germinating Cotton 1
- Chromatin and DNA synthesis associated with nuclear membrane in germinating cotton.
- Fat Utilization and Composition in Germinating Cotton Seeds. 12
- Metabolic Dysfunction and Unabated Respiration Precede the Loss of Membrane Integrity during Dehydration of Germinating Radicles1
- The Development of Isocitric Lyase Activity in Germinating Cotton Seed 1
