Mechanism of Oxidative Carbon Dioxide Production during Renilla reniformis Bioluminescence

AUTOR(ES)

DeLuca, Marlene

RESUMO

The oxidation of luciferin catalyzed by sea pansy luciferase results in the emission of light. Molecular oxygen is required and carbon dioxide is produced. When the reaction occurs in the presence of H218O, both of the oxygens of the carbon dioxide are labeled. One of the oxygens arises from the nonenzymic exchange of the ketone group of the substrate; the other oxygen is incorporated during the enzymic oxidation of the luciferin. When the reaction is carried out in the presence of 18O2, neither of the oxygens of the carbon dioxide is labeled. Thus the source of oxygen in the carbon dioxide is water. A mechanism for the oxidative reaction is proposed.

Documentos Relacionados

• OXYGEN CONSUMPTION AND CARBON DIOXIDE PRODUCTION DURING THE GROWTH OF YEAST
• Isolation and expression of a cDNA encoding Renilla reniformis luciferase.
• STUDIES ON CARBON DIOXIDE V. THE MECHANISM RESPONSIBLE FOR THE PRESERVING ACTION OF CARBON DIOXIDE ON DIPHTHERIA TOXIN1
• ON THE MECHANISM OF CARBON DIOXIDE REPLACEMENT IN HETEROTROPHIC METABOLISM1
• Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM 1