Melting during steady-state transcription of the rrnB P1 promoter in vivo and in vitro.
AUTOR(ES)
Ohlsen, K L
RESUMO
The rRNA rrnB P1 promoter was probed with the single-strand-selective reagent potassium permanganate during steady-state transcription in vitro and in vivo. In both cases, a weak but significant level of permanganate sensitivity was observed, which was not changed by treatment with rifampin. In contrast, static studies showed that rifampin strongly affects the very high level signal associated with polymerases that have used ATP and CTP as initiating nucleotides. We infer that the permanganate sensitivity associated with steady-state transcription is due to polymerases that have not yet used ATP and CTP. The slow and regulated step during rrnB P1 transcription may be the use of the initiating nucleotides to catalyze stable opening of the promoter DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=207672Documentos Relacionados
- Transcription of the Escherichia coli rrnB P1 promoter by the heat shock RNA polymerase (E sigma 32) in vitro.
- Growth rate-dependent control of the rrnB P1 core promoter in Escherichia coli.
- Localization of the intrinsically bent DNA region upstream of the E.coli rrnB P1 promoter.
- UP element-dependent transcription at the Escherichia coli rrnB P1 promoter: positional requirements and role of the RNA polymerase α subunit linker
- Both fis-dependent and factor-independent upstream activation of the rrnB P1 promoter are face of the helix dependent.