Messenger RNA stability in Saccharomyces cerevisiae: the influence of translation and poly(A) tail length.

AUTOR(ES)

Santiago, T C

RESUMO

A comparison between the half-lives of 10 specific yeast mRNAs and their distribution within polysomes (fractionated on sucrose density gradients) was used to test the relationship between mRNA translation and degradation in the eukaryote Saccharomyces cerevisiae. Although the mRNAs vary in their distribution across the same polysome gradients, there is no obvious correlation between the stability of an mRNA and the number of ribosomes it carries in vivo. This suggests that ribosomal protection against nucleolytic attack is not a major factor in determining the stability of an mRNA in yeast. The relative lengths of the poly(A) tails of 9 yeast mRNAs were analysed using thermal elution from poly(U)-Sepharose. No dramatic differences in poly(A) tail length were observed amongst the mRNAs which could account for their wide ranging half-lives. Minor differences were consistent with shortening of the poly(A) tail as an mRNA ages.

Documentos Relacionados

• Poly(A) Tail Length Control in Saccharomyces cerevisiae Occurs by Message-Specific Deadenylation
• Heat Shock Disrupts Cap and Poly(A) Tail Function during Translation and Increases mRNA Stability of Introduced Reporter mRNA.
• Poly(A) tail shortening is the translation-dependent step in c-myc mRNA degradation.
• Post-transcriptional modification of the poly(A) length of galactose-1-phosphate uridyl transferase mRNA in Saccharomyces cerevisiae.
• mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation