Metabolic and inflammatory evaluation in fiber and fructooligosaccharides supplemented diet in normal colon and trinitrobenzene sulphonic acid-induced colitic rats / Efeitos da suplementação com fibra solúvel ou frutooligossacarídeos sobre a inflamação e o metabolismo no colo normal ou na vigência de colite induzida por ácido trinitrobenzeno sulfônico, em ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of this study was research the metabolic and inflammatory findings in normal colon and trinitrobenzene sulphonic acid-induced (TNBS) colitic rats which received soluble fiber and fructooligosaccharides-FOS. Ninety six Wistar male rats were used, average weight 300g. They were distributed in two groups, of 48 animals each. One group with animals without colitis (GESC) submitted to transanal water enema and another group with colitis (GEC) produced by TNBS, 20mg. Each group was distributed into three subgroups, according to the used substance: 1(water), 2(soluble fiber), 3(FOS). All the animals received 3 ml such substances twice daily into the stomach through an intragastric catether during 14 days before the transanal water enema and the colitis production until the euthanasia day (7th and 14th. Days). The animals were evaluated concerning to the weight, colon weight/size relationship, macroscopic and microscopic scores and the metabolites colon in vivo concentrations (ketone bodies [3-hydroxybutirate, acetoacetate], piruvate, lactate and ATP). The cell redox estate was also evaluated by the [acetoacetate]/[3-hydroxybutirate] and [piruvate]/[lactate] relationship. GESC and GEC were evaluated, equally the subgroups water from the group without colitis (ESC1) comparing to the colitis group (EC1). No significant differences were found in the various inflammatory evaluated parameters in GESC group. An increased colon weight/size relationship was found in GEC group in the subgroup FOS, on the 14th day (p<0,05). Concerning to the animals weight from the subgroup GEC1, a significant decreasing was found on the 7th day. An increased colon weight/size relationship was found in macroscopic and microscopic scores in the subgroup GEC1 on the 7th and 14th days (p<0,05). Concerning to the metabolic evaluation, an increased acetoacetate concentration was found in GESC, in the fiber subgroup on the 14th day (p<0,05). Decreased piruvate concentrations in the fiber and FOS subgroups were demonstrated on the 14th day (p<0,05). The ATP concentrations were reduced in the fiber and FOS subgroups on the 7th and 14th days (p<0,05). The piruvate concentrations were increased in GEC group, in the fiber subgroup, on the 14th day (p<0,05). The ATP concentrations decreased in the fiber and FOS subgroups (p<0,05). Comparing the subgroups GESC1 with GEC1, an increased cetonic corps concentration was found in GEC1 on the 7th and 14th days (p<0,05). There was reduction of piruvate levels in GEC1 on the 7th and 14th days (p<0,05). There was also reduction in acetoacetate/3-hidroxibutirate and piruvate/lactate relationship in GEC1, on the 7th day (p<0,05). Its concluded that the soluble fiber or FOS intragastric intake doesnt change the evaluated inflammatory parameters on the 7th and 14th days in normal colons or during colitis process, but it increases the precursors offering for energy production, modifying the ATP in vivo concentrations in normal colonic tissue and during colitis process.

ASSUNTO(S)

colitis Ácido trinitrobenzenossulfônico inflamação colite diet in fiber fibra na dieta cirurgia proctologica colon - metabolism rat rato colo - metabolismo

ACESSO AO ARTIGO

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