Metabolism of the herbicide bromoxynil by Klebsiella pneumoniae subsp. ozaenae.
AUTOR(ES)
McBride, K E
RESUMO
Enrichment of soil samples for organisms able to utilize the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) as a nitrogen source yielded bacterial isolates capable of rapidly metabolizing this compound. One isolate, identified as Klebsiella pneumoniae subsp. ozaenae, could completely convert 0.05% bromoxynil to 3,5-dibromo-4-hydroxybenzoic acid and use the liberated ammonia as a sole nitrogen source. Assays of cell extracts of this organism for the ability to produce ammonia from bromoxynil revealed the presence of a nitrilase (EC 3.5.51) activity. The enzyme could not utilize 3,5-dibromo-4-hydroxybenzamide as a substrate, and no 3,5-dibromo-4-hydroxybenzamide could be detected as a product of bromoxynil transformation. Comparison of related aromatic nitriles as substrates demonstrated that the Klebsiella enzyme is highly specific for bromoxynil.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=203524Documentos Relacionados
- Cerebral abscess caused by Klebsiella ozaenae.
- Cloning and expression in Escherichia coli of a Klebsiella ozaenae plasmid-borne gene encoding a nitrilase specific for the herbicide bromoxynil.
- Nucleotide sequence of the gene encoding the SHV-2 beta-lactamase (blaSHV-2) of Klebsiella ozaenae.
- Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae.
- Infections caused by Klebsiella ozaenae: a changing disease spectrum.
